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E Roc1b RING domain is unable to interact with the same E2's as the Roc1a RING domain when assembled into Cullin complexes, which would suggest that at least one essential target of a Roc1a E3 ligase is dependent on the specific RING domain sequence of Roc1a. The AN2R construct was also unable to rescue Roc1aG1 lethality (data not shown), consistent with the failure of the AN2R protein to bind Cul
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We used laboratory-bred B. schlosseri colonies, offspring of founders collected at Monterey, Half Moon Bay and Moss Landing, CA, USA, and Banyuls, France marinas. The animals were maintained on glass slides at 20uC, in a flow-through facility at the National Institute of Oceanography, Haifa as described [15]. Large colonies were routinely subcloned into ramets of different sizes that were then tra
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We used laboratory-bred B. schlosseri colonies, offspring of founders collected at Monterey, Half Moon Bay and Moss Landing, CA, USA, and Banyuls, France marinas. The animals were maintained on glass slides at 20uC, in a flow-through facility at the National Institute of Oceanography, Haifa as described [15]. Large colonies were routinely subcloned into ramets of different sizes that were then tra
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